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r spondin1 conditioned medium  (R&D Systems)


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    Structured Review

    R&D Systems r spondin1 conditioned medium
    R Spondin1 Conditioned Medium, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r+spondin1+conditioned+medium/Recombinant+Mouse+R-Spondin+1+Protein%2C+CF/pm41275500-305-9-17
    Average 95 stars, based on 128 article reviews
    r spondin1 conditioned medium - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis
    Article Snippet: Briefly, stomachs removed from mice were minced with surgical scissors and processed by Liberase TH (Roche Diagnostics). .. The processed cells were suspended in Matrigel (Corning), plated onto 48-well multiple-well plates, and cultured in WRC+ medium, which consisted of Advanced DMEM/F-12 medium (Invitrogen) supplemented with GlutaMAX-I (Invitrogen), HEPES (Invitrogen), Penicillin-Streptomycin (Invitrogen), B-27 and N-2 supplement (Invitrogen), N-Acetylcysteine (Sigma-Aldrich), Valproic acid (Sigma-Aldrich), Recombinant murine EGF and Noggin (Peprotech), Afamin/Wnt3a CM (MBL Life Science), Chir99021 (R&D Systems), Recombinant human FGF10 (Peprotech), Y-27632 and SB431542 (R&D Systems), R-Spondin1 conditioned medium (generated from Cultrex HA-R-Spondin1-Fc 293T cell line; R&D Systems). .. The organoids were cultured for 5 to 7 days and passaged using TrypLE Express (Invitrogen).

    Recombinant:

    Article Title: Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis
    Article Snippet: Briefly, stomachs removed from mice were minced with surgical scissors and processed by Liberase TH (Roche Diagnostics). .. The processed cells were suspended in Matrigel (Corning), plated onto 48-well multiple-well plates, and cultured in WRC+ medium, which consisted of Advanced DMEM/F-12 medium (Invitrogen) supplemented with GlutaMAX-I (Invitrogen), HEPES (Invitrogen), Penicillin-Streptomycin (Invitrogen), B-27 and N-2 supplement (Invitrogen), N-Acetylcysteine (Sigma-Aldrich), Valproic acid (Sigma-Aldrich), Recombinant murine EGF and Noggin (Peprotech), Afamin/Wnt3a CM (MBL Life Science), Chir99021 (R&D Systems), Recombinant human FGF10 (Peprotech), Y-27632 and SB431542 (R&D Systems), R-Spondin1 conditioned medium (generated from Cultrex HA-R-Spondin1-Fc 293T cell line; R&D Systems). .. The organoids were cultured for 5 to 7 days and passaged using TrypLE Express (Invitrogen).

    Article Title: Maturation of human intestinal epithelial cell layers fortifies the apical surface against Salmonella attack.
    Article Snippet: .. This medium is based on DMEM/F12 supplemented with 5% R-Spondin1-conditioned medium (made from Cultrex 293T R-spondin1expressing cells; R&D Systems), 10% Noggin-conditioned medium (made with HEK293-mNoggin-Fc cells; kindly provided by Hans Clevers, Utrecht University), 50ng/ml mouse recombinant EGF (Sigma-Aldrich), 1xB27 supplement (Thermo Fisher Scientific/Gibco), 1.25mM N-acetyl cysteine (Sigma-Aldrich), and 100U/ml penicillin-streptomycin. .. For IEC differentiation toward an M-cell-like phenotype, ENRRT medium was used, generated by supplementing the ENR medium with 200ng/ml Recombinant Mouse TRANCE (RANKL) (BioLegend) and 50ng/ml Recombinant Human TNF (ProSci).

    Article Title: High-Definition DIC Imaging Uncovers Transient Stages of Pathogen Infection Cycles on the Surface of Human Adult Stem Cell-Derived Intestinal Epithelium
    Article Snippet: .. The medium for differentiation consisted of DMEM-F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells; R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells; kindly provided by Hans Clevers, Utrecht University), 50 ng/ml mouse recombinant EGF (Sigma-Aldrich), 1× B27 supplement (Gibco), 1.25 mM N -acetyl cysteine, and 100 U/ml penicillin-streptomycin ( , ). ..

    Generated:

    Article Title: Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis
    Article Snippet: Briefly, stomachs removed from mice were minced with surgical scissors and processed by Liberase TH (Roche Diagnostics). .. The processed cells were suspended in Matrigel (Corning), plated onto 48-well multiple-well plates, and cultured in WRC+ medium, which consisted of Advanced DMEM/F-12 medium (Invitrogen) supplemented with GlutaMAX-I (Invitrogen), HEPES (Invitrogen), Penicillin-Streptomycin (Invitrogen), B-27 and N-2 supplement (Invitrogen), N-Acetylcysteine (Sigma-Aldrich), Valproic acid (Sigma-Aldrich), Recombinant murine EGF and Noggin (Peprotech), Afamin/Wnt3a CM (MBL Life Science), Chir99021 (R&D Systems), Recombinant human FGF10 (Peprotech), Y-27632 and SB431542 (R&D Systems), R-Spondin1 conditioned medium (generated from Cultrex HA-R-Spondin1-Fc 293T cell line; R&D Systems). .. The organoids were cultured for 5 to 7 days and passaged using TrypLE Express (Invitrogen).



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    Image Search Results


    Journal: STAR Protocols

    Article Title: An optimized protocol for isolation of murine pancreatic single cells with high yield and purity

    doi: 10.1016/j.xpro.2024.102836

    Figure Lengend Snippet:

    Article Snippet: R-Spondin1-conditioned medium , Trevigen , 3710-001-K.

    Techniques: Recombinant, Lysis, Sterility

    Mouse Complete Feeding Medium—Organoids

    Journal: STAR Protocols

    Article Title: An optimized protocol for isolation of murine pancreatic single cells with high yield and purity

    doi: 10.1016/j.xpro.2024.102836

    Figure Lengend Snippet: Mouse Complete Feeding Medium—Organoids

    Article Snippet: R-Spondin1-conditioned medium , Trevigen , 3710-001-K.

    Techniques: Concentration Assay